Figure 3.
TLR2-dependent secretion of interleukins by B cells. Purified spleen B-cells derived from BALB/c (black bars), C57BL/6 WT (white bars) or C57BL/6 TLR2(−/−) (dashed bars) mice were cultured in 96-well plates at 1 × 106/ml in the absence or the presence of LPS (1 μg/ml) or SIR2RP1 (1 or 10 μg/ml). After 72 hr of incubation, the levels of TNF-α (a) and IL-12p40 (b) were measured in the culture supernatants by ELISA. The results shown are one representative of two different experiments that yielded similar results. *P < 0·05, **P < 0·01.