Table 1.

Nucleotide sequences

tsCD1d primers
cDNA	F0: 5′ TCACCGGATGTACTGATAGAAGCAGC 3′
	R0: 5′ ATGGGGT(AG)CCT(AG)C(CT)GT(GT)(CGT)CTG 3′
Wild type	F1: 5′ CCCAAGCTTGTCCCGCAAAGGCATTTCC 3′
	R1: 5′ GTCGAGGATCCCGGATGTACTGATAGAAGC 3′
Mutation Q86K	F2: 5′ GACGTACAGGAATTCGCCAAAATG 3′
Mutation H89R	F3: 5′ CAGGAATTCGCCCAAATGATACGCTTAGTC 3′
huCD1d primers
Wild type	F1: 5′ CCCAAGCTTAGGCTTTTCCCCCTCCGC 3′
	R1: 5′ CGGGATCCAGGACGCCCTGATAGGA 3′
Mutation K86Q	F2: 5′ GACGTGAAGGAATTCGCCCAAATG 3′
Mutation R89H	F3: 5′ AAGGAATTCGCCAAAATGCTACACTTATCC 3′
CD5-FLAG sequence
	5′GCTAGCTTCTAGAGTCCCTCGACCTCGAGATCCATTGTGCTCTAAAGGAGATACCCGGCCAGACACCCTCACCTGCGGTGCCCAGCTGCCCAGGCTGAGGCAAGAGAAGGCCAGAAACCATGCCCATGGGGTCTCTGCAACCGCTGGCCACCTTGTACCTGCTGGGGATGCTGGTCGCTTCCTGCCTCGGACGÿGCTAGCTTCTAGAGTCCCTCGACCTCGAGATCCATTGTGCTCTA 3′