cDNA |
F0: 5′ TCACCGGATGTACTGATAGAAGCAGC 3′ |
|
R0: 5′ ATGGGGT(AG)CCT(AG)C(CT)GT(GT)(CGT)CTG 3′ |
Wild type |
F1: 5′ CCCAAGCTTGTCCCGCAAAGGCATTTCC 3′ |
|
R1: 5′ GTCGAGGATCCCGGATGTACTGATAGAAGC 3′ |
Mutation Q86K |
F2: 5′ GACGTACAGGAATTCGCCAAAATG 3′ |
Mutation H89R |
F3: 5′ CAGGAATTCGCCCAAATGATACGCTTAGTC 3′ |
huCD1d primers |
Wild type |
F1: 5′ CCCAAGCTTAGGCTTTTCCCCCTCCGC 3′ |
|
R1: 5′ CGGGATCCAGGACGCCCTGATAGGA 3′ |
Mutation K86Q |
F2: 5′ GACGTGAAGGAATTCGCCCAAATG 3′ |
Mutation R89H |
F3: 5′ AAGGAATTCGCCAAAATGCTACACTTATCC 3′ |
CD5-FLAG sequence |
|
5′GCTAGCTTCTAGAGTCCCTCGACCTCGAGATCCATTGTGCTCTAAAGGAGATACCCGGCCAGACACCCTCACCTGCGGTGCCCAGCTGCCCAGGCTGAGGCAAGAGAAGGCCAGAAACCATGCCCATGGGGTCTCTGCAACCGCTGGCCACCTTGTACCTGCTGGGGATGCTGGTCGCTTCCTGCCTCGGACGÿGCTAGCTTCTAGAGTCCCTCGACCTCGAGATCCATTGTGCTCTA 3′ |