Figure 2.

TimEX-seq. (A) Comparison of TimEX results obtained using tiling arrays or massively parallel sequencing (basophilic erythroblasts). The S/G₁ ratio of the frequency of uniquely matched reads in 5-kb windows was calculated and smoothed as in Figure 1. (X-axis) Genomic distances; (y-axis) S/G₁ ratio for sequencing, and log₂ (S/G₁) for tiling arrays. Sequencing and tiling arrays produce very similar profiles. (B) Coefficient of correlation between tiling array and sequencing results. (C) Comparison of TimEX results obtained using tiling arrays or massively parallel sequencing (hESC). (D, top) Examples of chromosome-wide TimEX-seq profiles obtained based on 10.5 million (basophilic erythroblasts) and 13 million reads (hESC). A sigma of 100 kb was used for the Gaussian convolution. (Bottom) Differential timing curve for hESC and erythroid cells obtained by subtracting the hESC profile from the erythroid profile.