Skip to main content
. 1998 Sep 1;95(18):10493–10498. doi: 10.1073/pnas.95.18.10493

Figure 2.

Figure 2

p107 interacts with the LXCXE-like motif of HDAC1 (A) The IACEE sequence of HDAC1 is required for p107 binding. In vitro-translated, labeled HDAC1 (lanes 1–4) or HDAC1 ΔIACEE (lanes 5–8) was subjected to a GST pull-down analysis by using beads covered with the indicated GST fusion protein. (B) LXCXE motif-containing peptides inhibit p107/HDAC1 interaction. In vitro-translated labeled HDAC1 was subjected to a GST pull-down analysis by using beads covered with GST (lane 2) or GST-p107 fusion protein. Before GST pull-down analysis, beads were incubated without (lane 3) or with 15 μg of control (lane 4), SV40 T (lane 5), or HDAC1 (lane 6) peptide.