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. 1998 Sep 1;95(18):10493–10498. doi: 10.1073/pnas.95.18.10493

Figure 3.

Figure 3

p107 and p130 interact with a cellular histone deacetylase. (A) p107 is associated physically with a histone deacetylase. Nuclear extracts from U937 cells were immunoprecipitated with an anti-p107 (p107) or anti-HA (control) antibodies. Immunoprecipitates then were assayed for the presence of histone deacetylase activity. (B) p130 is associated physically with a histone deacetylase. Whole cell extracts from Jurkat cells were immunoprecipitated with anti-p130 (p130) or anti-HA (control) antibodies. Immunoprecipitates then were assayed for the presence of histone deacetylase activity. (C) p107 interacts with a cellular histone deacetylase through an LXCXE-like motif. Nuclear extracts from Jurkat cells were subjected to a GST pull-down analysis with beads covered with the indicated GST fusion protein. Before the incubation with nuclear extracts, beads were incubated with 100 μg of SV40 T peptide where indicated or 100 μg of control peptide. Bound proteins were assayed for the presence of deacetylase activity.