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. 1998 Sep 1;95(18):10493–10498. doi: 10.1073/pnas.95.18.10493

Figure 5.

Figure 5

p107 and p130 repress E2F4 activity through a histone deacetylase. (A) SAOS2 cells were transfected transiently with 2 μg of E2F-luc reporter vector, 100 ng of pcDNA3 myc-E2F4, 100 ng of pCMV DP2, and the indicated dose of pCMV p107. The amount of CMV promoter was kept constant by using empty vectors. TSA (100 ng/ml) was added (gray bars) or not (black bars) 8 h later, and luciferase activity was assayed 24 h after transfection. Result of a typical experiment is shown on the Left. The mean of fold repression by p107 calculated from various independent experiments in the absence (circles) or in the presence (squares) of TSA is shown on the Right. (B) SAOS2 cells were transiently transfected and assayed as in A with the indicated dose of CMV p130.