Fig. 6.
Analyses of OspC and RpoS production in the antibody escape ospAB mutants. Strains B313, B314 and B313 and B314 complemented with a wild-type copy of ospAB that are designated B313/pOspBAB and B314/pOspAB, respectively (labelled on top), were cultivated at either 23°C or 35°C. Whole-cell lysates were subjected to SDS-PAGE (Coomassie blue stain) (A–C) and immunoblot (D) analyses. For immunoblotting, a mixture of monoclonal antibodies directly against FlaB, RpoS and OspC was used. The bands corresponding to OspA, OspB, OspC, FlaB and RpoS were labelled on the right.