Figure 3.

Depletion of integrin β1 or PRL-3 abrogates PRL-3-promoted metastasis in vivo. LoVo-P cells were infected with lentivirus interfering with PRL-3, integrin β1, or control respectively at a multiplicity of infection value of 100 for 48 h, or left untreated. (A) Validation of silencing efficiency of Lentivirus by RT-PCR and Western blot. (B) Each nude BALB/c mouse was injected via the tail vein with 2.5 × 10⁶ LoVo-C or LoVo-P cells. Two months later, mice were sacrificed, and 4-μm paraffin slices of liver and lung tissues were stained with hematoxylin and eosin dyes and examined under a light microscope. Arrows indicate the presence of a metastatic tumor in a lung slice.