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. Author manuscript; available in PMC: 2009 Dec 12.
Published in final edited form as: Transgenic Res. 2006 Jun;15(3):393–397. doi: 10.1007/s11248-006-0008-1

Figure 1.

Figure 1

Gnmt gene targeting. (a) Knockout strategy for replacing the first exon and promoter region of gnmt by the neomycin resistance gene (neo) from the targeting vector pPNT. The vertical bars represent exons 1–6. (b) Genotyping by PCR with P1, P2 and P3 primers of the mice from F2 generation after crossing gnmt+/− mice. M, molecular size markers; WT, wild type mouse; TC, target construct. (c) Levels of GNMT in liver crude extracts. Western blotting was performed after separation of 15 μg of total proteins from liver crude extracts. Rabbit antibodies against rat GNMT were used in the first step and goat IgG conjugated with peroxidase used as secondary antibodies. St, GNMT marker; M, male; F, female animals. (d) Enzyme activity. Values are means +/− S.E.M. Each group was significantly different from the others.