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. 1998 Sep 1;95(18):10505–10510. doi: 10.1073/pnas.95.18.10505

Figure 2.

Figure 2

Intracellular ssDNA accumulated in S. aureus. Total DNA was isolated from plasmid-containing cultures, electrophoresed on 0.7% agarose gels, and transferred to nitrocellulose paper with (A) or without (B) prior denaturation. The Southern blots were probed and subjected to autoradiography. The positions of the supercoiled (SC), open circular (OC), and single-stranded (SS) forms of plasmid DNA are indicated.