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In vitro replication of ssDNA in S. aureus extracts. ssDNA containing the pLS1 ssoA (pA-pLS1ssoA) or the pE194 ssoA (M13-pE194ssoA) was replicated for 60 min, and the reaction products were linearized with HindIII (H) or EcoRV (E). The reaction products were analyzed by agarose gel electrophoresis. The vector pALTER (pA) ssDNA was used as the negative control. The amounts of cell-free extract (Ext) used are indicated.
