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RNA polymerase-directed synthesis of primer RNAs. ssDNA containing the pLS1 ssoA (pLssoA) and pE194 ssoA (pEssoA) in the functional (+) or nonfunctional (−) orientation were used as template. Reactions were carried out by using the S. aureus (S.au) or B. subtilis (B.su) RNA polymerase. A mixture of labeled oligonucleotides were used as size markers (M). The sizes of the major primer RNA products from the pE194 ssoA (17 nt) and the pLS1 ssoA (20 nt) are indicated by arrows.
