Skip to main content
. 1998 Sep 1;95(18):10505–10510. doi: 10.1073/pnas.95.18.10505

Figure 6.

Figure 6

Determination of the 5′-end of the primer RNAs. In vitro-synthesized primer RNAs were used as a template for primer extension analysis. The positions of a major 17-nt band observed with the pE194 ssoA (pEssoA) and a 18-nt product obtained with the pLS1 ssoA (pLssoA) are indicated by arrowheads. Their sizes were corrected for the presence of a phosphate (31) in the mixture of labeled oligonucleotides markers (M). In samples 1 and 3, S. aureus RNA polymerase was used for the synthesis of RNA primers, whereas the B. subtilis RNA polymerase was used in sample 2.