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. 2009 Dec 12;213(1):118–127. doi: 10.1242/jeb.037077

Fig. 3.

Fig. 3.

RT-PCR profiling of H. americanus calcitonin-like diuretic hormone (Homam-CLDH) in the pacemaker neuron- and motor neuron-specific sub-regions of the lobster cardiac ganglion (CG). (A) Schematic representation of the lobster CG showing the location of the pacemaker and motor neurons within the ganglion. (B) RT-PCR profiling of CLDH message in the pacemaker (lane 1) and motor neurons (lane 2) of the cardiac ganglion. A robust band of the predicted CLDH PCR product size (496 bp in length; base pair ladder shown in lane 4) was consistently detected only in the motor neurons of the ganglion. No PCR product was dectected in the negative control (lane 3).