Table 2.
Vector- and insert-specific primers used for sequencing the Homarus americanus calcitonin-like diuretic hormone (CLDH) cDNA
| Primer name | Direction | Sequence |
| Vector-specific primers | ||
| SP6 | Forward | ATTTAGGTGACACTATAG |
| T-Party | Reverse | TTTTTTTTTTTTTTTTTTTV |
| Insert-specific primers | ||
| CLDH F1 | Forward | GTGGTGGACCCAACATCAGTGACG |
| CLDH F2 | Forward | GTTGGAGTTGCTGACGAGATTGG |
| CLDH F3 | Forward | CAGGAGACCATCTTGTTAACTG |
| CLDH F4 | Forward | TCCAGACAACGACGGTCAACCG |
| CLDH R1 | Reverse | CCAATCTCGTCAGCAACTCCAAC |
| CLDH R2 | Reverse | CTAGCTCATTGGCCCTTATGATGGAGTGCC |
Abbreviations: V in T-Party represents A, T or G.