Fig. 6.
Isoflurane reduces synaptic UNC-13::GFP puncta. Representative images of segments of dorsal nerve cord from unc-13(s69);nuIs46[UNC-13S::GFP;ccGFP] L4 larval animals treated with (A) Air, no PMA (phorbol-12-myristate-13-acetate), (B) isoflurane (2 vol%), no PMA, (C) Air, 1 μg/ml PMA, (D) isoflurane (2 vol%), 1 μg/ml PMA. Note synaptic puncta (example denoted by arrowhead) decreased in B and D relative to their respective air controls. scale bar = 20 μm.