Table 1.
Strains list
| Strain | Genotype | Mutation | Transforming plasmid | Transforming protein | Refs. |
|---|---|---|---|---|---|
| N2 | wild type | None | None | None | 16 |
| MC339 | unc-64(md130) | Truncated syntaxin1-227 +few novel aa + reduced wild type syntaxin | None | None | 8,13 |
| MC270 | md130 +/+ bli-5 | heterozygous md130 products | None | None | 8,13 |
| MC72 | unc-64(md130); gcEx5 | Truncated syntaxin1-227 +few novel aa + reduced wild type syntaxin | pTXfull-length = pTX21; pPH::GFP-1 | Full-length wild type UNC-64; hypodermal GFP | 8,13 |
| MC185 | gcEx85 | None | pTXmd130; pPH::GFP-1 | md130 product; hypodermal GFP | 21 |
| MC105 | gcEx95 | None | pTX1-258; pPH::GFP-1 | Truncated syntaxin residues 1-258; hypodermal GFP | 21 |
| MC153, MC155 | gcEx53, gcEx55 | None | pTX1-227; pPH::GFP-1 | Truncated syntaxin residues 1-227; hypodermal GFP | 21 |
| MC150, MC151, MC152, MC184, MC187 | gcEx50, gcEx51, gcEx52, gcEx84, gcEx87 | None | pTX1-158; pPH::GFP-1 | Truncated syntaxin residues 1-158; hypodermal GFP | 21 |
| MC139, MC158, MC159, MC232 | gcEx91, gcEx58, gcEx59, gcEx90 | None | pTX1-106; pPH::GFP-1 | Truncated syntaxin residues 1-106; hypodermal GFP | 21 |
| MC233, MC234, MC271 | gcEx92, gcEx93, gcEx94 | None | pTX1-86; pPH::GFP-1 | Truncated syntaxin residues 1-86; hypodermal GFP | 21 |
| MC176, MC177, MC178 | gcEx76, gcEx77, gcEx78 | None | pTX1-64; pPH::GFP-1 | Truncated syntaxin residues 1-64; hypodermal GFP | 21 |
| NM1968 | slo-1(js379 null) | Loss of SLO-1 BK channel | None | None | 9,26 |
| PS1762 | goa-1(sy192 lf) | Dominant negative Go-alpha protein | None | None | 11 |
| VC223 | tom-1(ok285 null) | Loss of tomosyn | None | None | 17 |
| EG1985 | unc-64(js115 null); oxIs34 | Loss of syntaxin | Punc-64(L166A/E167A); Pmyo-2::GFP | Open Syntaxin; pharynx GFP | 18 |
| MC272 | tomo-1(ok285 null); unc-64(js115 null); oxIs34 | Loss of tomosyn; Loss of syntaxin | Punc-64(L166A/E167A); Pmyo-2::GFP | Open Syntaxin; pharynx GFP | 17,18 |
| MC261 | tomo-1(ok285 null) unc-13(e376);unc-64(js115 null);oxIs34 | Loss of tomosyn; Reduction of UNC-13; Loss of syntaxin | Punc-64(L166A/E167A); Pmyo-2::GFP | Open Syntaxin; pharynx GFP | 17,18,19 |
| MC275 | tomo-1(ok285 null) unc-13(s69); unc-64(js115 null); oxIs34 | Loss of tomosyn; Loss of UNC-13; Loss of syntaxin | Punc-64(L166A/E167A); Pmyo-2::GFP | Open Syntaxin; pharynx GFP | 17,18,19 |
| MC344 | unc-10(md1117 null); unc-64(js115 null); oxIs34 | Loss of UNC-10 - RIM; Loss of syntaxin | Punc-64(L166A/E167A); Pmyo-2::GFP | Open Syntaxin; pharynx GFP | 45 |
| CB55 | unc-2(e55 null) | Loss of non-L-type Ca++ Channel | None | None | 29,36 |
| BC168 | unc-13(s69 null) | Loss of UNC-13 | None | None | 19 |
| CB376 | unc-13(e376 lf) | Reduction of UNC-13 | None | None | 19 |
| KP3299 | unc-13(s69 null); nuIs46 | Loss of UNC-13 | KP268[UNC-13S::GFP]; ccGFP | UNC-13S::GFP; coelomocyte GFP | 19,23 |
| EG2840 | unc-13(s69 null); oxIs78 | Loss of UNC-13 | KP280[myr::unc-13S::GFP]; ccGFP | Myristoylated UNC-13S::GFP; coelomocyte GFP | 19,20 |
| RM2333 | mdIs3 | None | C44E1; Psnb-1::GFP - integrant of mdEx43 | wild type UNC-13; neuronal GFP | 19 |
| MC273 | mdIs3;gcEx37 | None | C44E1; Psnb-1::GFP; pTXmd130; pPH::GFP-1 | wild type UNC-13; neuronal GFP; md130 truncated syntaxin; hypodermal GFP | 19,21 |
| MC274 | mdIs3;gcEx58 | None | C44E1; Psnb-1::GFP; pTX1-106; pPH::GFP-1 | wild type UNC-13; neuronal GFP; truncated syntaxin 1-106; hypodermal GFP | 19,21 |
More than one strain in a row represents multiple transformants with the same plasmid; If - loss-of-function but not necessarily null Is - indicated a chromosomally integrated array, Ex - indicates an extrachromosomal array; aa = amino acid