Fig. 4.

Antibodies to Hp-CTL-1 react with H. polygyrus excretory–secretory antigens (HES). Polyclonal antibodies raised in rat against recombinant Hp-CTL-1 were tested by ELISA for reactivity with the homologous immunogen Hp-CTL-1 (A), an unrelated recombinant protein containing a hexa-histidine tag, Bm-Galectin (B), and HES (C). Normal rat serum controls are shown in each panel. (D) Positive control of rat anti-HES serum tested by ELISA on HES. Data shown are means ± SD of duplicate determinations at each dilution, and similar results were obtained with two different polyclonal antisera raised against Hp-CTL-1.