Figure 6. Fusion or hemifusion of membrane model systems promoted by rBPI₂₁.

LUV labeled with 0.6% mol of RhB-PE and NBD-PE were mixed with unlabeled liposomes to a final proportion of 1∶4 and the energy transfer assay was evaluated in the presence of rBPI₂₁ after 10 min of incubation. Bars represent the range from three independent experiments. POPC (black circles); POPG (blue circles); POPC∶POPG 55∶45 (orange circles); POPC∶POPG 80∶20 (green circles); POPC∶LPS 80∶20 (red circles); POPC∶POPG∶LPS 60∶20∶20 (brown circles). The differences in (hemi)fusion percentage for rBPI₂₁ 9 µM were statistically significant between all data pairs, except POPC/POPC∶LPS (80∶20).