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. 2009 Dec 22;4(12):e8385. doi: 10.1371/journal.pone.0008385

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Domingues et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Carboxyfluorescein fluorescence increase measured 20 min after rBPI₂₁ addition in POPC (black circles), POPC∶LPS 80∶20 (red circles), POPC∶POPG 80∶20 (green circles), POPC∶POPG 55∶45 (orange circles), POPC∶POPG∶LPS 60∶20∶20 (brown circles) and POPG (blue circles) liposomes. Bars represent the range from at least two independent experiments. The differences in leakage percentages for rBPI₂₁ 9 µM were statistically significant for the following data pairs: POPC/POPC∶LPS (80∶20), POPC/POPC∶POPG (80∶20), POPC/POPC∶POPG (55∶45), POPC/POPG, POPC/POPC∶POPG∶LPS (60∶20∶20), POPC∶LPS (80∶20)/POPG∶POPG (55∶45), POPC∶LPS (80∶20)/POPG, POPC∶POPG (80∶20)/POPC∶POPG (55∶45), POPC∶POPG (80∶20)/POPG, POPC∶POPG (55∶45)/POPC∶POPG∶LPS (60∶20∶20) and POPC∶POPG∶LPS (60∶20∶20)/POPG.