Figure 5.

KD of Notch1 gene expression in normal SVZ neurosphere cells decrease neurosphere cell proliferation. A shows real-time PCR assay for Notch1. B shows Western blot assay for Notch1, NICD, and Hes1. C shows quantitative data of Ki67 expression. DI show Ki67 immunostaining in normal, scrambled, or Notch1-KD SVZ neurosphere cells treated with or without atorvastatin (blue color is the DAPI nuclear counterstain; D and G, normal neurosphere cells treated with or without atorvastatin; E and H, scrambled neurosphere cells treated with or without atorvastatin; F and I, Notch1-KD neurosphere cells treated with or without atorvastatin).