Fig. 5.
Treatment of cell cultures with anti-interleukin (IL)-10 antibody restored interferon (IFN)-γ production in the cultures of cells from the group with parasitized placenta. Cord blood mononuclear cells (CBMC) from parasitized (upper figure, black bars; n = 10) and non-parasitized (lower figure, grey bars; n = 12) placenta were either depleted of regulatory T cells (Tregs-) or left untreated and cultured in the presence of anti-IL-10 antibody or an isotype control antibody. Following stimulation with live Plasmodium falciparum Pf164-infected red blood cells, IFN-γ release was measured. Significant IFN-γ recovery was observed after regulatory T cell depletion (P1 = 0·014) and anti-IL-10 blocking antibody (P2 = 0·021). Results are shown as the mean ± standard error of the mean (minus backgrounds).