Fig. 2.

Effect of HE2000 on numbers of polymorphonuclear cells and exudate volume in murine model of carrageenan (CAR)-induced pleurisy. Male CD-1 mice (n = 8 per group) were anaesthetized and either saline alone (0·1 ml) or saline containing 2% λ-CAR (0·1 ml) was injected into the pleural cavity. Mice were treated subcutaneously with HE2000, vehicle or intraperitoneally with anti-tumour necrosis factor-α antibody 24 h and 1 h before CAR challenge. At 4 h after the injection of CAR, the pleural cavity rinsed with 1 ml of saline solution. The exudate and washing solution were removed by aspiration. The leucocytes in the exudate were counted with an optical microscope in a Burker's chamber after vital Trypan blue staining. All values in the figures are expressed as mean ± standard deviation (s.d.). Similar studies were performed twice and yielded similar results. *Significant (P < 0·0001) difference compared to control (vehicle) values.