Fig. 3.

Effect of HE2000 on exudate levels of interleukin (IL)-6 in the murine model of carrageenan (CAR)-induced pleurisy. Male CD-1 mice (n = 2–4 per group) were anaesthetized and either saline (0·1 ml) or saline containing 2% λ-CAR (0·1 ml) was injected into the pleural cavity. Mice were treated subcutaneously with HE2000 (40 mg/kg) or with vehicle 24 h and 1 h before CAR challenge. At 2–8 h after the injection of CAR, the pleural cavity was rinsed with 1 ml of saline solution. The exudate and washing solution were removed by aspiration. Levels of IL-6 were measured by enzyme-linked immunosorbent assay. Data are expressed as pg/ml ± standard deviation. Differences trended (P = 0·06) in favour of HE2000 treatment in terms of reduced IL-6. Similar studies were performed twice and yielded similar results.