Fig 3.
(A) Internalization of PNATAR-CPP in HIV-1 virions. The HIV-1 virions incubated with I125-labeled PNATAR-CPP conjugate was subjected to discontinuous sucrose density gradient ultracentrifugation. Fractions collected from the bottom and analyzed for p24 antigen and for radioactivity [35].
(B) Abortive endogenous reverse transcription in HIV-1 virions pretreated with PNATAR-CPP conjugate [35]. Lane1, untreated HIV-1 virions; lanes 2 and 3, pre-treated with 1 μM and 2.5 μM of PNATAR Transportan conjugate, respectively; lanes 4 and 5, pre-treated with scrambled PNA-Transportan conjugate at 2 μM and 5 μM concentrations, respectively.