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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Expert Opin Biol Ther. 2009 Aug;9(8):975–989. doi: 10.1517/14712590903052877

Figure 5.

Figure 5

(A) PNATAR.Neamine specifically cleaves the target RNA but not the corresponding DNA. Internally 32P-labeled TAR RNA or 5′ 32P-labeled DNA incubated in the presence or absence of 400 nM 5-neamine-PNATAR conjugate at 25°C. Lanes 1 through 4 in each panel show the results after incubation periods of 0, 1, 3, and 5 h, respectively [54].

(B). PNATAR-Neamine Conjugate cleaves HIV-1 TAR RNA in the vicinity of its binding site. The internally 32P-labeled HIV-1 TAR RNA incubated without or with 400 nM of the conjugate at room temperature. Lanes 1-4 represent incubation for 0, 1, 3 and 5 h, respectively. The arrow indicates the possible cleavage site on TAR RNA generating approximately 60-70 nucleotide long products [33].