Sedimentation Equilibrium.
(A) Absorbance versus radial position for 45 μM W223A btnOH-AMP at 22,000 (◊), 26,000 (×) and 30,000 (○) rpm obtained in standard buffer at 20 °C. The best-fit curves were generated from global analysis using a monomer-dimer model of six data sets acquired at multiple loading concentrations and centrifuged at three speeds. The residuals of the fit for each data set shown are provided in the bottom panels.
(B) Free energy of dimerization, ΔGoDim, of each ABL variant complexed with btnOH-AMP or bio-5’-AMP (dark bars) compared to that measured for the wild type repressor (white bars).