Table 4.
BirA variant | No ligand (apo) (min-1) | bio-5’-AMP (holo) (min-1) | kapo/kholo |
---|---|---|---|
Wild Type | 0.026 ± 0.003 | 0.004 ± 0.001 | 6.5 ± 0.3 |
V219A | 0.032 ± 0.007 | 0.013 ± 0.004 | 2.5 ± 0.4 |
V214A | 0.018 ± 0.006 | 0.007 ± 0.002 | 2.6 ± 0.4 |
W223A | 0.041 ± 0.010 | 0.008 ± 0.001 | 5.1 ± 0.3 |
All measurements were performed in Standard Buffer (10 mM Tris-HCl pH 7.50 ± 0.02 at 20.0 ± 0.1 °C. 200 mM KCl, 2.5 mM MgCl2) as described in Materials & Methods. The reported rates represent the average of at least two independent determinations and the errors represent the 95% confidence intervals.