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. Author manuscript; available in PMC: 2009 Dec 14.
Published in final edited form as: J Mol Biol. 2007 Jul 26;373(1):96–111. doi: 10.1016/j.jmb.2007.07.020

Table 4.

Pseudo-first Order Rates of Proteolytic Cleavage of ABL Variant Proteins

BirA variant No ligand (apo) (min-1) bio-5’-AMP (holo) (min-1) kapo/kholo
Wild Type 0.026 ± 0.003 0.004 ± 0.001 6.5 ± 0.3
V219A 0.032 ± 0.007 0.013 ± 0.004 2.5 ± 0.4
V214A 0.018 ± 0.006 0.007 ± 0.002 2.6 ± 0.4
W223A 0.041 ± 0.010 0.008 ± 0.001 5.1 ± 0.3

All measurements were performed in Standard Buffer (10 mM Tris-HCl pH 7.50 ± 0.02 at 20.0 ± 0.1 °C. 200 mM KCl, 2.5 mM MgCl2) as described in Materials & Methods. The reported rates represent the average of at least two independent determinations and the errors represent the 95% confidence intervals.