Figure 5.

Footprints on the viral DNA ends of various MLV mutants. All complexes were salt-stripped and reconstituted with BAF followed by MM-PCR. Characteristic enhancements are located at ≈20 bp and ≈100 bp from the U3 end, and ≈20 bp from the U5 end. Shown are footprints on the U3 (A) and U5 (B) viral DNA ends. (a) Control MLV preintegration complexes made by coculturing clone 4 with NIH 3T3 cells. (b-d) Preintegration complexes obtained by transient transfection of 293T cells with plasmid (b) pNCA (wild-type MLV); (c) pSQ70 (active dl8276–22 MLV); and (d) pJM1X1 (inactive dl8269–7 MLV). Reconstitution was done in the absence of BAF in lanes 1 and with 2 ng BAF in lanes 2.