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Phenomenological rate constant (kiobs) and relative amplitude (Ai%) of each kinetics phase obtained by fitting the stopped-flow kinetics presented in Figure 4a and Figure 5 to a tri-exponential function.
| k1obs | k2obs | k3obs | A1% | A2% | A3% | |
|---|---|---|---|---|---|---|
| L7W (pH 6.5) | 282 ± 29 | 11 ± 1 | 2.0 ± 0.1 | 56 ± 5 | 10 ± 2 | 34 ± 2 |
| T21W (pH 6.5) | 217 ± 10 | 25 ± 7 | 1.4 ± 0.1 | 49 ± 6 | 31 ± 4 | 20 ± 2 |
| L7W/H9V (pH 6.5) | 174 ± 10 | 8 ± 1 | 2.2 ± 0.6 | 31 ± 1 | 11 ± 2 | 58 ± 3 |
| T21W (pH 7.5) | 956 ± 50 | 39 ± 3 | 1.3 ± 0.2 | 54 ± 6 | 30 ± 4 | 16 ± 2 |
