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. Author manuscript; available in PMC: 2010 Oct 15.
Published in final edited form as: Dev Biol. 2009 Aug 3;334(2):409–417. doi: 10.1016/j.ydbio.2009.07.039

Fig. 5.

Fig. 5

Expression of pyloric sphincter (PS) markers is aberrant in the Six2-null stomach. (A) Nkx2.5 is expressed in the E12.5 wild-type PS territory (arrow). (B) However, in the prospective PS territory of E12.5 Six2−/− littermates, the domain of Nkx2.5 expression (arrow) is smaller. (C) At E14.5, Nkx2.5 expression remains in the wild-type presumptive PS territory (arrow). (D) Nkx2.5 expression appears normal in the Six2-null stomach at this stage. Sox9 is expressed in the mesenchyme of the prospective PS region (arrows) and in the epithelium of the stomach (arrowheads) at E12.5 (E) and E14.5 (G). In the absence of Six2, the domain of mesodermal cells (arrows) expressing Sox9 is smaller at E12.5 (F) and absent at E14.5 (H); its pattern of expression is normal in the stomach epithelium at both stages (arrowheads). Gremlin is also expressed in the mesenchyme of the presumptive wild-type PS region (arrows) at E12.5 and E14.5 (I, K). The territory of mesenchymal cells expressing Gremlin (arrows) is smaller in the Six2−/− stomach at E12.5 (J) and E14.5 (L). Scale bars, 100 µm.