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Results for the in vitro splicing assay. The wild-type (Wt) and mutant (Mt) minigenes L1-GHRexon8-L2 and Adml-par were incubated in HeLa nuclear extracts for 60 min at 30 °C. Control reaction mixtures were incubated at 0 °C. The structures of the pre-mRNA and the mRNA splice products are indicated next to the autoradiogram. The expected size of the splice products is also indicated. Bands corresponding to correct and aberrant mRNA splice products are indicated by the arrows.
