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Effects of the GHR T to A nucleotide change on mRNA splicing in HEK293 cells. (A) Radioactive RT-PCR analysis performed with mRNA from HEK293 cells transfected with the wild-type or mutant minigene L1-GHRexon8-L2. The structure of the correct (L1-Ex8-L2) and aberrant (L1–L2) splice products and their expected sizes are indicated. (B) Schematic representation of the splice events for the wild-type and mutant minigenes. Mutation location is indicated by an asterisk.
