Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Dec 21;4(12):e8387. doi: 10.1371/journal.pone.0008387

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Khare et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

K_m values for substrates and V_max values were determined by performing FadD13 assays as described in the section on “Materials and Methods”. For the calculation of Michaelis constants, a varying range of substrate concentrations (Palmitic acid – 1.5–60 µM, ATP - 0.03–4 mM, CoenzymeA - 0.02–1.5 mM) were employed and the K_m values were calculated by GraphPad Prism 5 (San Diego, California, USA) by using a non-linear fitting method. (A) Comparison of V_max values of the native FadD13 (NP) and its mutants. K_m values of Palmitic acid (B), ATP (C) and CoenzymeA (D) for native FadD13 (NP) and its mutants. The data is depicted as mean of values ± S.E. of two independent experiments carried out in duplicates.