Figure 2. 2D map and identification of P. acanthamoebae immunogenic proteins.

A. Proteins reacting with at least one rabbit anti-Parachlamydia serum were excised from gel and analysed by MALDI TOF mass spectrometry. Spots successfully identified are numbered. Molecular mass standards are indicated on the right side of the gel. B. The potential of 18 immunogenic proteins for use in a serological diagnostic test was evaluated based on their reactivity with control sera and on their sequence similarity in BLASTP results (see Table S2 for detailed analysis).