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. 2009 Dec 23;4(12):e8427. doi: 10.1371/journal.pone.0008427

Figure 1. Expression, localization, and suppression of α-actinin isoforms in glioma cells.

Figure 1

(A) Localization of α-actinin-1 and α-actinin-4 in U-373 MG cells cultured on collagen-coated glass. Solid and open arrowheads mark the localization of individual α-actinin isoforms at ruffles and along actin stress fibers, respectively. Scale Bar = 10 µm. (B) Measurement of siRNA efficacy by Western Blot of U-373 MG cells transfected with either scrambled control siRNA (siCTL) or siRNA directed against α-actinin-1 (siACTN1) or α-actinin-4 (siACTN4) (*p<0.05). (C) Subcellular localization of α-actinin-1 and α-actinin-4 following siRNA treatment. Arrowheads depict the redistribution of α-actinin-4 to FAs when α-actinin-1 is suppressed. Scale Bar = 20 µm.