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. 2009 Dec 23;4(12):e8427. doi: 10.1371/journal.pone.0008427

Figure 6. Effect of α-actinin expression on myosin expression and contractility.

Figure 6

(A) Effect of α-actinin suppression on NMMII expression by Western Blot. NMMII expression is significantly lower (*p<0.05) in both α-actinin-1 and α-actinin-4-depleted cells than in controls. (B) Effect of α-actinin depletion on traction force generation measured by traction force microscopy. Box-whisker plots of root-mean squared (RMS) traction of siCTL, siACTN1 and siACTN4 cells on 2 kPa and 18 kPa ECM substrates. The error bars mark the maximum and minimum of the data sets, the three horizontal lines mark the 25th, 50th, and 75th percentile of the data, and the point marks the mean. N>10 cells in all cases.