Figure 5. Expression of CD25 and CD122 by CD4⁺ cells in the liver of chronic mice challenged with T. cruzi trypomastigotes.

C57BL/6 mice infected for 7 months with T. cruzi were challenged i.v. with 5×10⁶ trypomastigotes and, after 24 and 48 h, the liver leukocytes analyzed by flow cytometry. (A) Expression of CD25 and CD122 by CD4⁺ cells. Frequency of large cells (B) and total liver cell numbers (C) of CD4⁺ subpopulations defined by expression of CD25 and CD122 molecules. Numbers in dot blots indicate the mean frequencies of cells in quadrants. Numbers in histograms indicate the mean frequencies of large cells among cell subsets defined by quadrants in A. Data in C correspond to the mean±SD of total liver cell numbers of the indicated subpopulations. A representative experiment (n = 3) of two is shown. Differences between groups were evaluated by ANOVA and Tukey's multiple comparison tests (* p<0.01; ** p<0.001, compared to chronic mice).