Table 1. Summary of the combinations of tumor cells and sera used for mapping the serospecificities of cutaneous lymphoma patients by 2-dimensional electrophoresis and proteome analyses.

Tumor cells† *	Sera‡ *	Number of antigens#
		total detected	assigned	identified
Heterogeneous combinations
MyLa	A: 1, 4, 5, 7, 8, 9, 11, 12	29	12	6
MyLa	B: 14, 18, 19, 20, 21, 22, 23, 24	12	6	0
MyLa	C: 24	4	0	0
MyLa	D: 12	11	9	2
Autologous combinations
Patient 85	85	38	13	13
Patient 86	86	12	12	9
Patient 87	87	3	0	0
Total		109	52	30

^†The tumor cells used for the 2-dimensional electrophoreses were either the mycosis fungoides tumor cell line MyLa or tumor cells freshly isolated from tumor lesion of the indicated patients.

^‡The sera used to identify tumor-associated antigens by 2-dimensional Western blot analyses were obtained from patients with cutaneous lymphoma. For the heterogeneous combinations of sera and tumor cells, 8 independent sera each were grouped into two Western blot experiments. In 2 cases single sera were analyzed. The Western blots A–D are shown in Figure 2.

^*The numbering of the sera in this summary is the same as indicated in Figure 1 for the 1-dimensional Western blot analyses and Figures 2–4 for the 2-dimensional analyses.

^#The numbers given for the antigens refer to the indicated Western blot analyses. Some antigens were identified repeatedly for different tumor cells and patients.