Table 2. Antigens identified for cutaneous lymphoma by proteome serology.
| Spot Nr. | Antigen (gene identifier numbers) | Mw [kDa] | pI | Number of masses† | Sequence coverage† | Number of antigen spots‡ | |
| total | matched | ||||||
| Heterogeneous combinations | |||||||
| 3 | BiP (gi|6470150) | 70.9 | 5.23 | 29 | 24 | 42% | 1 |
| 5 | HSP 60 (gi|49522865) | 61.2 | 5.70 | 43 | 25 | 48% | 1 |
| 6 | HSP 71 (gi|32467) | 70.9 | 5.37 | 85 | 37 | 50% | 2 |
| 7 | HSP 60 (gi|77702086) | 61.2 | 5.70 | 48 | 14 | 28% | 1 |
| 8 | β tubulin (gi|57209813) | 47.7 | 4.70 | 39 | 19 | 34% | 1 |
| 9 | mt-ATP Synthase, β-subunit (F1 complex) (gi|16741373) | 56.5 | 5.26 | 35 | 22 | 42% | 1 |
| 15 | TIP47 (gi|16306789) | 47.0 | 5.30 | 32 | 14 | 44% | 1 |
| Autologous combinations | |||||||
| 1 | aconitase (gi|20072188) | 85.51 | 7.62 | 46 | 25 | 31% | 1 |
| 2 | β tubulin (gi|567053) | 49.70 | 4.78 | 57 | 13 | 33% | 1 |
| 3 | coronin (gi|5902134) | 51.00 | 6.25 | 58 | 18 | 28% | 1 |
| 4 | glutamate dehydrogenase (gi|20151189) | 61.36 | 7.66 | 53 | 27 | 42% | 1 |
| 5 | keratin 16 (gi|24430192) | 51.21 | 4.99 | 63 | 14 | 30% | 1 |
| 6 | lamin A/C isoform 1 (gi|27436946) | 75.00 | 6.57 | 61 | 30 | 43% | 1 |
| 7 | lamin A/C isoform 2 (gi|5031875) | 65.01 | 6.40 | 61 | 40 | 56% | 1 |
| 8 | lamin B1 (gi|5031877) | 66.37 | 5.13 | 29–99 | 24–69- | 34–43% | 2 |
| 9 | vimentin (gi|5030431, gi|340234) | 35.02–56.55 | 4.82–5.06 | 41–95 | 12–51- | 29–74% | 13 |
The antigens are listed with reference to the antigen spot numbers as indicated in Figures 2 and 4, their masses and isoelectric points and the outcomes of the peptide mass fingerprints analyses.
The spot numbers refer to the numbering given for the proteome-serological analyses for the heterogeneous and autologous combinations of tumor cells and sera, respectively.
The numbers of peaks detected, numbers of peaks assigned and sequence coverage are the results of the peptide mass fingerprint analyses by MASCOT. In case of TIP47 a number of calculated fragments are di-, tri- or tetrapeptides with masses below 500 Dalton which set as threshold in this case.
The number of antigen spots refers to all analyses reported herein. Numbers higher than 1 indicate that the same antigen was identified repeatedly in independent protein spots, some in independent electrophoresis gels, vimentin repeatedly in independent 2 gels.