Fig. 2.

PEDF has no effect on late-stage differentiation in 3T3-L1 preadipocytes. A: schema indicating that PEDF (10–100 nM) was added on days 3 and 5 during differentiation. B: intracellular lipid was stained by Oil Red O on day 7. B, a: nondifferentiated cells; B, b: MDI; B, c: MDI + 10 nM PEDF; B, d: MDI + 100 nM PEDF. C: quantification of lipid content by spectrophotometer. D–F: expression of adipocyte marker proteins aP2 (D), ATGL (E), and HSL (F) on day 7 by Western blot analysis. D–F, top: representative blots. D–F, bottom: quantification by densitometry. Results represent 3 independent experiments (means ± SD).