Fig. 4.

Effect of PEDF on expression of CCAAT/enhancer-binding protein-α (C/EBPα) and peroxisome proliferator-activated receptor-γ (PPARγ). PEDF (10–100 nM) was added to postconfluent 3T3-L1 preadipocytes on day 0 (A and B) or to the differentiating cells on day 3 (C and D). Expression of C/EBPα (A and C) and PPARγ (B and D) was determined by Western blot analysis and quantified by densitometry. Results represent 3 independent experiments (means ± SD; n = 3). ‡P < 0.01 vs. nondifferentiated cells; *P < 0.05, **P < 0.01 vs. MDI (ANOVA).