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PEDF inhibits mitotic clonal expansion in 3T3-L1 preadipocytes. Postconfluent preadipocytes were treated with MDI in the presence or absence of PEDF (10–100 nM) for 72 h. Cellular DNA content was measured by CyQuant NF Cell Proliferation Assay Kit. Results represent 3 independent experiments (means ± SD). ‡P < 0.01 vs. nondifferentiated cells; *P < 0.05, **P < 0.01 vs. MDI (ANOVA).
