Fig. 8.

Expression of endogenous PEDF was downregulated during early adipogenesis in 3T3-L1 preadipocytes. A: mRNA levels of PEDF and Pref-1 in untreated or mature adipocytes (day 7) were determined by real-time RT-PCR and normalized by 18S RNA levels (means ± SD). **P < 0.01 (Student's t-test). B: PEDF secreted into the medium was measured by ELISA and normalized by cell number (means ± SD). **P < 0.01 (Student's t-test). C: PEDF mRNA expression was determined by real-time RT-PCR in differentiating cells during the first 24 h after MDI treatment. Results represent 3 independent experiments (means ± SD). *P < 0.05, **P < 0.01 (ANOVA).