Fig. 9.

GLN increases HSP70 and Sp1 promoter-reporter activity. Cells were transfected with either an HSP70 or an Sp1 reporter construct. Presence of 8 mM GLN during heat shock led to significant increase in both HSP70 and Sp1 promoter-driven heat-inducible luciferase activity, compared with HS CT (*P < 0.05 by Bonferroni multiple-comparisons test; n = 3). FuGENE represents cells not transfected with plasmid but treated with FuGENE transfection reagent alone, and NC represents luciferase activity in control plasmid-transfected cells (all post-heat stress). All groups were normalized to total protein content.