Fig. 4.

Effect of exogenous spermidine and spermine on electrophysiologic properties of normal BUC. A: representative whole cell I-V relationships recorded without (control) or with 100 μM spermidine or spermine in pipette solution in human BUC from normal control patients. Inset: normal (n = 3 subjects) and OAB (n = 3 subjects) whole cell I-V currents. B: resting outward current density measured at +50 mV potential. Spermine and spermidine inhibit the outward current density significantly, P < 0.05, n = 9. C: dose-response curves for spermidine (•) and spermine (○) obtained at +60 mV from inside-out patches. The polyamines were applied to the cytosolic side; n = 12–16 from five different subjects.