Table 1.
IFNγ-induced alterations in gene expression by quantitative RT-PCR
| Threshold Cycle |
||||||
|---|---|---|---|---|---|---|
| Gene | 0 h | 0.5 h | 2 h | 6 h | 15 h | 24 h |
| STAT1 | 26.2 | 25.8 | 22.1 | 21.0 | 21.3 | 21.2 |
| IRF-1 | 31.1 | 27.4 | 22.2 | 22.3 | 22.3 | 22.2 |
| IRF-2 | 27.6 | 27.6 | 26.0 | 25.6 | 26.0 | 26.1 |
| IRF-8 | ND | ND | 30.9 | 31.1 | 32.0 | 31.9 |
| IFNGR1 | 26.4 | 26.4 | 26.4 | 26.3 | 26.5 | 26.3 |
| IFNGR2 | 26.0 | 26.0 | 25.1 | 24.7 | 25.1 | 25.2 |
| SOCS3 | 26.9 | 25.4 | 24.6 | 24.0 | 24.8 | 24.9 |
| PIAS1 | 27.0 | 26.9 | 27.2 | 27.1 | 26.8 | 26.7 |
| NOS2A | ND | 39.1 | 35.9 | 31.6 | 32.7 | 33.5 |
| CFTR | 38.0 | 36.9 | 37.3 | 38.5 | ND | 37.9 |
GAPDH was used for data normalization. IRF, interferon regulatory factor; IFNGR1 and IFNGR2, IFNγ receptor subunits 1 and 2; SOCS3, suppressor of cytokine signaling 3; PIAS1, protein inhibitor of activated STAT1; NOS2A, inducible nitric oxide synthase; ND, not determined.