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. 2009 Oct 7;297(6):C1424–C1433. doi: 10.1152/ajpcell.00324.2009

Fig. 1.

Fig. 1.

Stimulation with 5-hydroxytryptamine (5-HT) suppresses the activity of Cdc42GAP in smooth muscle cells. A: Cdc42GAP immunoprecipitated from 5-HT (10 μM, 15 min) stimulated cells or unstimulated cells was added to preloaded Cdc42 for 10 min. GTPase activating protein (GAP) activity was then determined using the method described in materials and methods. Representative blots showing that higher amount of GTP-Cdc42 treated with Cdc42GAP precipitates from stimulated cells compared with unstimulated cells, suggesting that 5-HT stimulation inhibits the activity of Cdc42GAP in smooth muscle cells. B: amounts of GTP-Cdc42 treated with Cdc42GAP precipitates are normalized to the level of preloaded GTP-Cdc42 (without exposure to GAP immunoprecipitates). *Significantly higher GTP-Cdc42 levels after treatment with GAP precipitates from stimulated cells than the levels from unstimulated cells (P < 0.05). Values are means ± SE (n = 4).