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. 2009 Oct 7;297(6):C1424–C1433. doi: 10.1152/ajpcell.00324.2009

Fig. 4.

Fig. 4.

Knockdown of p47phox disinhibits the activity of Cdc42GAP during 5-HT stimulation but not exposure to hydrogen peroxide. Cells producing luciferase shRNA or p47phox shRNA were stimulated with 10 μM 5-HT or 100 μM hydrogen peroxide for 15 min, or they were not stimulated. Cdc42GAP was immunoprecipitated from these cells, and GAP activity was then assessed. A: representative immunoblots showing the effects of p47phox shRNA on GAP activity during stimulation with 5-HT. Hydrogen peroxide-induced GAP suppression was not affected by knockdown of p47phox. B: amounts of GTP-Cdc42 treated with Cdc42GAP precipitates from cells producing luciferase shRNA or p47phox shRNA are normalized to the level of preloaded GTP-Cdc42. *Significantly higher GAP activity in response to 5-HT stimulation in cells producing p47phox shRNA than in cells expressing luciferase shRNA (P < 0.05). Values are means ± SE (n = 3–4).